DNA methylation plays important roles in gene regulation and is highly relevant to human diseases and cancer. The most common types of DNA methylation include 5-methylcytosine (5mC), N4-methylcytosine (4mC) and N6-methyladenine (6mA). Compared to 5mC, which is the most studied DNA methylation, 6mA and 4mC are at lower levels and only detectable by highly sensitive technologies. Recently developed single molecule, real-time (SMRT) sequencing technology can directly detect base modification by monitoring the kinetic variations of single bases, in which 6mA and 4mC provide sensitive signals of DNA methylation.
MethSMRT is an integrative database for DNA 6mA and 4mC methylomes, generated from SMRT sequencing. The database provides a platform to host, browse, search and download 6mA and 4mC profiles for 156 species. It also offers a genome browser to visualize DNA methylation sites and related information such as SNPs and gene annotation. Furthermore, the database provides a quick summary of methylome of 6mA and 4mC for each species.